Henry Hawksfield, joint master of the Crawley and Horsham Hunt, West Sussex, was filmed by hunt saboteurs at three meets in January and February this year.
The videos allegedly showed him using hounds to kill foxes and prompted a police investigation.
The 58-year-old from Partridge Green, near Horsham, Neil Millard, 44, from nearby Shipley, Rachel Holdsworth, from Washington, near Storrington and Andrew Phillis, 50, from Totnes in Devon, will all make their first appearance at Crawley magistrates on Thursday.
They have been charged under the Hunting Act 2004, which banned hunting all mammals with dogs.
Since the Act came into force seven years ago, only about 10 members of organised hunts have been prosecuted.
Anyone found guilty of a fox hunting offence can be fined up to £5,000 and have their hounds and vehicles confiscated.
The Crawley and Horsham Hunt, which has 70 members, refused to comment until after the case.
Four people are to appear in court in the first fox hunting prosecution in Sussex.
The quartet, who are all members of the Crawley and Horsham Hunt, are accused of illegally hunting a fox with a dog contrary to the Hunting Act 2004.
Sussex Police launched an investigation after videos allegedly showing a fox being killed during a hunt were passed to the force by members of the public.
Joint master of the Crawley and Horsham Hunt Henry James Hawksfield, 58, of Bines Road, Partridge Green, near Horsham, Rachel Holdsworth of Rock Road, Washington, near Storrington, Neil Millard, 44, of Dragons Lane, Shipley, near Horsham, and Andrew Phillis, 50, of Halwell, Totnes, in Devon, have been summonsed to appear at Crawley Magistrates’ Court to face four charges under Section 1 of the Hunting Act 2004.
They are due to appear in court on Thursday.
The offences are said to have occurred at meets of the Crawley and Horsham Hunt on January 18 in Southwater, on January 25 in Shermanbury and on February 15 in Twineham and Shermanbury.